KANPHOS, derived from the abbreviation of “kinase-associated neural phospho-signaling,” is an online database system that provides the phosphorylated sites identified by our recent studies as well as those previously reported in the literature. You will find which substrates are (including its phosphorylated sites) phosphorylated by a specific kinase and which extracellular stimuli activate (or inhibit) the protein phosphorylation via intracellular signaling cascades.
The database system and its web portal were built based on XooNIps. As of December 2021, about 6,200 pairs of protein kinases and phosphorylated sites identified by our method, as well as about 3,700 pairs cited from the literature, have been registered in the database. All data are controlled for quality via review and curation by specialists.
The web portal supports three modes of search:
Each protein (kinase/substrate) item is linked with external databases such as Uniprot KB (proteomics database), HGNC DB (human genomics database), HuGE Navigator (human genome epidemiology database), and Allen Brain Atlas, enabling us to easily predict unknown functions of the protein phosphorylation. As an advanced option, we also implemented a function to show a list of pathways in which the set of substrates phosphorylated by a specific condition is overrepresented more than expected, via communication with Reactome.
This study was supported by grants from MEXT andAMED (JP18dm0207005; JP21dm0207075; JP21wm0425017; JP21wm0425008), JSPS KAKENHI (JP16K18393, JP17H01380, JP17K07383, JP17H02220, JP17K19483, JP18K14849, JP19K16370, JP21K06428, JP21K06427), MEXT KAKENHI (JP17H05561, JP19H05209, JP21H00196); the Uehara Memorial Foundation; the Takeda Science Foundation; and the Hori Sciences and Arts Foundation.
KANPHOS is operated by International Center for Brain Science (ICBS), Fujita Health University, Toyoake, Japan.