KANPHOS, derived from the abbreviation of “kinase-associated neural phospho-signaling,” is an online database system that provides the phosphorylated sites identified by our recent studies as well as those previously reported in the literature. You will find which substrates are (including its phosphorylated sites) phosphorylated by a specific kinase and which extracellular stimuli activate (or inhibit) the protein phosphorylation via intracellular signaling cascades.
The database system and its web portal were built based on XooNIps. As of December 2021, about 6,200 pairs of protein kinases and phosphorylated sites identified by our method, as well as about 3,700 pairs cited from the literature, have been registered in the database. All data are controlled for quality via review and curation by specialists.
The web portal supports three modes of search:
- Search for substrates phosphorylated by a specific kinase;
- Search for kinases phosphorylating a specific protein; and
- Search for kinases and their target substrates by a specific signaling pathway.
Each protein (kinase/substrate) item is linked with external databases such as Uniprot KB (proteomics database), HGNC DB (human genomics database), HuGE Navigator (human genome epidemiology database), and Allen Brain Atlas, enabling us to easily predict unknown functions of the protein phosphorylation. As an advanced option, we also implemented a function to show a list of pathways in which the set of substrates phosphorylated by a specific condition is overrepresented more than expected, via communication with Reactome.
- Kozo Kaibuchi
- Junichiro Yoshimoto
- Mutsuki Amano
- Tomoki Nishioka
- Takayuki Kannon
- Taku Nagai
- Daisuke Tsuboi
- Yasuhiro Funahashi
- Yukie Yamashita
- Hisatsugu Koshimizu
This study was supported by grants from MEXT andAMED (JP18dm0207005; JP21dm0207075; JP21wm0425017; JP21wm0425008), JSPS KAKENHI (JP16K18393, JP17H01380, JP17K07383, JP17H02220, JP17K19483, JP18K14849, JP19K16370, JP21K06428, JP21K06427), MEXT KAKENHI (JP17H05561, JP19H05209, JP21H00196); the Uehara Memorial Foundation; the Takeda Science Foundation; and the Hori Sciences and Arts Foundation.
- Nagai T, Yoshimoto J, Kannon T, Kuroda K, Kaibuchi K. Phosphorylation Signals in Striatal Medium Spiny Neurons. Trends Pharmacol Sci. 2016 Oct;37(10):858-871. doi: 10.1016/j.tips.2016.07.003. [link]
- Ahammad RU, Nishioka T, Yoshimoto J, Kannon T, Amano M, Funahashi Y, Tsuboi D, Faruk MO, Yamahashi Y, Yamada K, Nagai T, Kaibuchi K. KANPHOS: A Database of Kinase-Associated Neural Protein Phosphorylation in the Brain. Cells. 2021 Dec 24;11(1):47. doi: 10.3390/cells11010047. [link]
KANPHOS is operated by International Center for Brain Science (ICBS), Fujita Health University, Toyoake, Japan.